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Solid-phase extraction combined with dispersive liquid–liquid microextraction has been developed as a new approach for the extraction of enrofloxacin in chicken meat prior to high performance liquid chromatography with UV detection. In the SPE-DLLME, enrofloxacin was first extracted from chicken meat into the mixture of 10 mL acetonitrile acidified with formic acid buffer (pH=4) and 10 mL EDTEA-McIlvaine buffer (0.1 M) and 20 mL n-hexane as an extracting phase by using ultrasound-assisted extraction. Then, the extract by ultrasound-assisted extraction was used for solid-phase extraction. After clean-up, enrofloxacin was preconcentrated by using DLLME technique. Thus, 1.5 mL methanol extract (disperser solvent) and 200 µL chloroform (extraction solvent) were added to 5.0 mL ultrapure water and a DLLME technique was applied. Under the optimum conditions, the linearity of the method was in the range from 10 to 500 µg kg-1 with the correlation coefficient (r2) of 0.9972. The method detection limit was 5.0 µg kg-1. The proposed method has been successfully applied to the analysis of the enrofloxacin in chicken meat, and a satisfactory result was obtained.