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This research was intended to investigate the connection between Knowledge Hiding and In-role Behavior. The relationship between independent and dependent variables was calculated by the Pearson correlation and simple regression analysis. The data was randomly collected through a self-administered questionnaire from 129 staff working in private sector universities.  This was a cross-sectional analysis and the findings showed that Knowledge Hiding negatively affect the in-role behavior of employees (r = 41.6, β = -.645, t = 9.51, p < 0.005). The results of this study suggested that universities need to focus on the Knowledge Hiding Behavior of employees in order to achieve their objectives and improve quality.