Find in Library

A biotrophic fungus, <i>Puccinia striiformis</i> f.sp. <i>tritici (Pst)</i>, which causes stripe rust disease in wheat is the most yield-limiting factor in wheat production. Plants have complex defense mechanisms against invading pathogens. Hypersensitive response (HR), a kind of programmed cell death (PCD) at the infection site, is among these defense mechanisms. Transcription factors (TFs) play a crucial role in plant defense response against invading pathogens. Myeloblastosis (MYB) TFs are among the largest TFs families that are involved in response to both biotic and abiotic stresses. However, little is known about the mechanisms of MYB TFs during the interaction between wheat and the stripe rust fungus. Here, we identified an R2R3 MYB TF from wheat, designated as <i>TaMYB391,</i> and characterized its functional role during wheat–<i>Pst</i> interaction. Our data indicated that <i>TaMYB391</i> is induced by <i>Pst</i> infection and exogenous application of salicylic acid (SA) and abscisic acid (ABA). <i>TaMYB391</i> is localized in the nucleus of both wheat and <i>Nicotiana benthamiana</i>. Transient overexpression of <i>TaMYB391</i> in <i>N. benthamiana</i> triggered HR-related PCD accompanied by increased electrolyte leakage, high accumulation of reactive oxygen species (ROS), and transcriptional accumulation of SA defense-related genes and HR-specific marker genes. Overexpression of <i>TaMYB391</i> in wheat significantly enhanced wheat resistance to stripe rust fungus through the induction of pathogenesis-related (PR) genes, ROS accumulation and hypersensitive cell death. On the other hand, RNAi-mediated silencing of <i>TaMYB391</i> decreased the resistance of wheat to <i>Pst</i> accompanied by enhanced growth of the pathogen. Together our findings demonstrate that <i>TaMYB391</i> acts as a positive regulator of HR-associated cell death and positively contributes to the resistance of wheat to the stripe rust fungus by regulating certain PR genes, possibly through SA signaling pathways.