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Green synthesis is a biocompatible and stable method of producing zinc oxide nanoparticles (ZnONPs).In the pre¬sent study, ZnONPs were biosynthesized using Fumaria parviflora extract by the green method, and the anti¬bacterial and antioxidant properties of these NPs were evaluated. The characteristics of the synthesized ZnONPs were determined by ultraviolet-visible spectrophotometry (UV-VIS), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The antioxidant activity of the NPs was tested by the α-diphenyl-β-picryl¬hydrazyl (DPPH) method. Antibacterial properties of the synthesized ZnONPs were evaluated against Staphylococcus aureus and Escherichia coli by disc diffusion and microdilution methods. The results of UV-VIS spectroscopy revealed an absorption peak at 370 nm. XRD results showed the formation of a hexagonal wurtzite structure, and SEM analyses demonstrated that ZnONPs had a spherical shape with an average size 42 to 60 nm. Free radical sca¬venging capacity of ZnONPs was assessed using the DPPH assay with varying concentrations of ZnONPs, and scavenging activity was observed with IC50 of 30.86 μg/ml. In the antibacterial assay, the inhibition zone of the synthesized NPs at 100 μg/ml concentration for S. aureus (24.6±0.72) was greater than that of the antibiotics vancomycin (23±0.51) and a mikacin (13±0.40) and was greater for E. coli (13.2±0.81)than that of vancomycin (12±0.41) (P ≤ 0.05). The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of ZnONPs for S. aureus and E. coli were 1.56 and 3.125 μg/ml, and 6.25 and 12.5 μg/ml, res¬pectively. The biosynthesized ZnONPs showed strong antibacterial and antioxidant activities.