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The quarantine insect pest <i>Phenacoccus solenopsis</i> (Hemiptera: Pseudococcidae) has a broad host range and is distributed worldwide. Each year, <i>P. solenopsis</i> causes significant crop losses. The detoxification of various xenobiotic compounds involves the cytochrome P450 monooxygenase (CYP) superfamily of enzymes. However, the functions of CYPs in <i>P. solenopsis</i> are poorly understood. In the present study, <i>P. solenopsis</i> was reared from the egg to the adult stage on three host plants: Tomato, cotton, and hibiscus. Thirty-seven <i>P. solenopsis</i> CYP genes were identified and their phylogenetic relationships were analyzed. Eleven CYP genes (<i>PsCYP4NT1</i>, <i>PsCYP4G219</i>, <i>PsCYP6PZ1</i>, <i>PsCYP6PZ5</i>, <i>PsCYP301B1</i>, <i>PsCYP302A1</i>, <i>PsCYP305A22</i>, <i>PsCYP315A1</i>, <i>PsCYP353F1</i>, <i>PsCYP3634A1</i>, and <i>PsCYP3635A2</i>) were selected for quantitative real-time PCR analysis. The results demonstrated marked differences in CYP expression levels in <i>P. solenopsis</i> grown on different host plants. The results will aid the molecular characterization of CYPs and will increase our understanding of CYP expression patterns in <i>P. solenopsis</i> during development and growth on different hosts.