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The wide distribution of bacteria and their highly dynamic activities have a great impact on the aquatic ecosystem. Surface water bodies constitute an important drinking water source that approximatively cover half of the worldwide need. Considering the main health threats, the microbial profile represents the most important indicator on the biology quality of water environments. One of the most important criterion is fecal indicators and in consequence the aim of this study was to monitor the seasonal fecal pollution variability in the surface water of Arges and Dambovita River during 2021. These rivers are part of the Arges-Vedea basin that cover 9% of Romania’s surface and they are located in the proximity of the Bucharest, the capital city of Romania.