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The aim of the present study was a rapid detection and identification of Enterococcus sp. in various segments of chicken gastrointestinal tract by polymerase chain reaction (PCR) analysis. As a biological material were used broiler chickens Hybro. They were fattening by the combined probiotic preparation for elimination of pathogens and better utilization of feed. In our study, the identification of Enterococcus species was based on the superoxid dismutase gene (sodA). Enterococcus faecium, Enterococcus faecalis were determined in all samples (100% occurence). Occurence of Enterococcus gallinarum was 87.5% and Enterococcus cecorum was 0%.