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In current investigation, morphological and biochemical characteristics of grape (Vitis vinifera L.) hybrids were evaluated at ICAR-IARI, New Delhi. Hybrids of Pearl of Csaba × Beauty Seedless came earliest in full bloom, maturity and harvest with largest size of bunch, while hybrids of Banqui Abyad × Beauty Seedless harvested last and produced smaller size of bunch. Larger berry size and maximum juice recovery was measured in hybrids Hy.16/2AR1P14 (Cardinal × Beauty Seedless) and Hy.16/2A-R4P13 (Banqui Abyad × Beauty Seedless). The maximum total soluble solids, TSS/acid ratio with least total titratable acidity was estimated in Pearl of Csaba × Beauty Seedless hybrids. Based on morphological and biochemical evaluation, two hybrids, i.e. Hy.ER-R2P36 and Hy.16/2A-R1P14 were found promising especially under subtropical conditions of North India.