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This study focuses on a crucial objective: eradicating the formidable dengue and filarial vectors through larval toxicity using a novel essential oil derived from Sphaeranthus indicus Linn. The research encompasses several key methodologies, including pest rearing, homology modeling, and molecular docking. The G Power value is taken as 0.8, with two groups and each group carrying 5 sample sizes. Si-CVO is toxic to Aedes aegypti larvae, and it causes dose-dependent mortality in the II, III, and IV instar larvae, respectively. Maximum mortality rates for all instars were 500 ppm: 91.3% (II instar), 87.4% (III instar), and 83.2%. (IV instar). While the lowest dosage of 100 ppm had a lower death rate throughout all larval instars. It is observed that by using the novel essential oil from Sphaeranthus indicus Linn which is a plant derived product the baleful insects are killed and the agriculture efficiency is improved.