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<i>MYC</i> is a major oncogene that plays an important role in cell proliferation in human cancers. Therefore, the mechanism behind <i>MYC</i> regulation is a viable therapeutic target for the treatment of cancer. Comprehensive and efficient screening of <i>MYC</i> regulators is needed, and we had previously established a promoter screening system using fluorescent proteins and the CRISPR library. For the efficient identification of candidate genes, a database was used, for which mRNA expression was correlated with <i>MYC</i> using datasets featuring “Similar” and “Not exactly similar” contexts. <i>INTS14</i> and <i>ERI2</i> were identified using datasets featuring the “Similar” context group, and <i>INTS14</i> and <i>ERI2</i> were capable of enhancing <i>MYC</i> promoter activity. In further database analysis of human cancers, a higher expression of <i>MYC</i> mRNA was observed in the <i>INTS14</i> mRNA high-expressing prostate and liver cancers. The knockdown of <i>INTS14</i> in prostate cell lines resulted in decreased <i>MYC</i> mRNA and protein expression and also induced G0/1 arrest. This study confirmed that CRISPR screening combined with context-matched database screening is effective in identifying genes that regulate the <i>MYC</i> promoter. This method can be applied to other genes and is expected to be useful in identifying the regulators of other proto-oncogenes.