Characterization of Dof Transcription Factors and the Heat-Tolerant Function of <i>PeDof-11</i> in Passion Fruit (<i>Passiflora edulis</i>)

oleh: Ge Chen, Yi Xu, Jie Gui, Yongcai Huang, Funing Ma, Wenhua Wu, Te Han, Wenwu Qiu, Liu Yang, Shun Song

Format: Article
Diterbitkan: MDPI AG 2023-07-01

Deskripsi

Abiotic stress is the focus of passion fruit research since it harms the industry, in which high temperature is an important influencing factor. Dof transcription factors (TFs) act as essential regulators in stress conditions. TFs can protect against abiotic stress via a variety of biological processes. There is yet to be published a systematic study of the Dof (<i>PeDof</i>) family of passion fruit. This study discovered 13 <i>PeDof</i> family members by using high-quality genomes, and the members of this characterization were identified by bioinformatics. Transcriptome sequencing and qRT-PCR were used to analyze the induced expression of <i>PeDof</i>s under high-temperature stress during three periods, in which <i>PeDof-11</i> was significantly induced with high expression. <i>PeDof-11</i> was then chosen and converted into yeast, tobacco, and Arabidopsis, with the findings demonstrating that <i>PeDof-11</i> could significantly respond to high-temperature stress. This research lays the groundwork for a better understanding of <i>PeDof</i> gene regulation under high-temperature stress.