Find in Library

Uncoordinated carbon-nitrogen ratio in raw materials will lead to excessive contents of higher alcohols in alcoholic beverages. The effect of <i>GAT1</i> gene, the GATA transcription activator, on higher alcohol biosynthesis was investigated to clarify the mechanism of <i>Saccharomyces cerevisiae</i> regulating higher alcohol metabolism under high concentrations of free amino nitrogen (FAN). The availability of FAN by strain SDT1K with a <i>GAT1</i> double-copy deletion was 28.31% lower than that of parent strain S17, and the yield of higher alcohols was 33.91% lower. The transcript levels of the downstream target genes of <i>GAT1</i> and higher alcohol production in the double-copy deletion mutant suggested that a part of the effect of <i>GAT1</i> deletion on higher alcohol production was the downregulation of <i>GAP1</i>, <i>ARO9</i>, and <i>ARO10</i>. This study shows that GATA factors can effectively regulate the metabolism of higher alcohols in <i>S. cerevisiae</i> and provides valuable insights into higher alcohol biosynthesis, showing great significance for the wheat beer industry.