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Tussen-die-Riviere Nature Reserve falls within the Eastern Mixed Nama-Karoo Biome. The surface area is approximately 23 000 ha and it is located in a summer rainfall region. Sixty six different mammal species were recorded for the reserve over the past 13 years which constitutes 65 % of the indigenous mammals recorded for the Free State Province. The highest number of species for the rodents and insectivores were recorded in riverine habitat but the highest abundance were recorded in rocky habitat and was constituted by only one species, Aethomys namaquensis.