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The extent of genetic diversity among 17 seed sources of Jatropha curcas was studied using 13 ISSR primers along with itscombinations. The number of bands produced by 13 individual primers ranged from 1 to 10. The percentage polymorphism orpolymorphic percentage ranges from 100 to 33.3 and the PIC value varied from 0.89 to 0.65. Among 13 ISSR primers, twoprimers viz., 895 and 899 produced 100% polymorphism.These genotypes were also tested with 78 combination primers for itsamplification. Out of seventy eight combinations, eight combinations were found to exhibit 100% polymorphism and its PIC valueranged from 0.86 to 0.38. The genetic similarity index was used to construct a dendrogram which illustrated that the 17genotypes fall into five clusters at 0.72 Jaccard’s similarity coefficient and the same data was used to construct the radial treeusing Darwin software. Both these software identified the two genotypes CJC 1 and CJC 19 as diverse. These diverseaccessions can be utilized in breeding programme to obtain more variability.