Find in Library

A novel tobacco(nicotiana tabacum) sucrose synthase genewas characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. Thissucrose synthase gene mRNA was 2,954bp in length containing a 2,418bp open reading framewhich encodes a protein of 805 amino acids. Sequence analysis revealed that thissucrose synthase of tobacco shares high homology with the sucrose synthase of Nicotianaattenuata(98%), Nicotianasylvestris(98%), Nicotianatomentosiformis(98%), Capsicumannuum(95%), Capsicumbaccatum(95%), Solanumtuberosum(94%), Solanumlycopersicum(94%) and Solanumpennellii(94%). Results also showed that tobacco sucrose synthase gene has a closer genetic relationship with the sucrose synthase gene of Capsicumannuum.Genomic DNA analysis indicated that tobacco sucrose synthase gene is structured in 13 exons and 12 introns.Tissue expression profile analysis revealed that this tobacco sucrose synthase gene was highly expressed in leaf, but moderately expressed in root, stemand flower. These established the foundation for further research on the tobacco sucrose synthase gene.