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Objective <i>Campylobacter</i> species are the main causes of foodborne illness worldwide, posing significant threats to public health. This study aimed to investigate the antibiotic resistance and genomic characterization of <i>C. jejuni</i>/<i>C.coli</i> from retail chickens in Beijing. Methods Antimicrobial susceptibility testing was conducted on 126 <i>C. jejuni</i>/<i>C. coli</i> isolated from retail chickens in Beijing, following CLSI protocols. Whole genomes of all isolates were sequenced using the Illumina platform. Results More <i>C. coli</i> (83.82%) showed multi-drug resistance than <i>C. jejuni</i> (8.62%). Genomic analysis demonstrated 42 sequence types (STs) and 12 clonal complexes (CCs), from which CC828 and CC52 were dominant. <i>cdtA</i>, <i>cdtB</i> and <i>cdtC</i> encoding cytotoxic protein were present spontaneously in most <i>C. jejuni</i> but not found in any <i>C. coli</i> isolates. The abundances of antibiotic resistance genes (ARGs) and virulence genes (VGs) in <i>C. jejuni</i> and <i>C. coli</i> were significantly different, with ARGs numbered in <i>C. coli</i> and VGs in <i>C. jejuni</i>. Conclusions High prevalence of multi-drug resistance <i>C. coli</i> and <i>C. jejuni</i> isolated from Beijing chickens were challenging clinical antibiotic usages in the treatment of <i>Campylobacter</i> infection. The surveillance of particular <i>C. jejuni</i> and <i>C. coli</i> STs correlated with higher resistance and virulence needs to be strengthened in the future.