Generation of a murine monoclonal antibody to capsular polysaccharide Vi from Salmonella Typhi
oleh: Fátima Reyes-López, Frank Camacho-Casanova, Oscar Otero-Alfaro, Maribel Cuello-Pérez, Darcy Núñez-Martínez, Sonsire Fernández-Castillo, Luis Guillermo García-Imía
| Format: | Article |
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| Diterbitkan: | Finlay Ediciones 2015-11-01 |
Deskripsi
The conventional hybridoma technology has enabled the development of monoclonal antibodies (Mabs) against many antigens. Mabs have several applications in the field of basic research, diagnosis, immunotherapy and vaccine manufacturing processes. Mabs-producing hybridomas against the capsular polysaccharide from <i>Salmonella</i> Typhi were obtained, after intraperitoneal immunization of BALB/c mice with 10 µg of capsular polysaccharide Vi conjugated to diphtheria toxoid, and subsequent fusion of lymphocytes isolated of the spleen and myeloma cells SP2/O. A Mab was selected, partially characterized, and named as 4G3E11. The isotype of this Mab was IgG1. It was proved by means of a sandwich ELISA that the 4G3E11 Mab reacts with different concentrations of polysaccharide in samples of the vax-TyVi® vaccine. The Mab obtained in this research could be useful as reagent for the detection and quantitation of polysaccharide Vi in typhoid vaccines.