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Ten large flowered chrysanthemum cultivars, viz., 'Beat Rice May', 'Beauty', 'Casa Grande', 'Jet Snow', 'John Weber' , 'Miss Maud Jeffries', 'Penny Lane', 'Shanker Dayal', 'Snow Ball' and 'S.S. Arnold' from National Botanical Research Institute (NBRI), Lucknow, were evaluated for morphological and biochemical characterization. Morphological data were recorded on vegetative and floral characters. Biochemical characterization included analysis of anthocyanins, carotenoides, chlorophyll content (chlorophyll a, b and total) and flavonoids. Results on morphological and biochemical parameters clearly indicated distinctness among cultivars with reference to differences in morphological characters and chemical composition of pigments.