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In today’s scenario, a number of nanocomposites are evidencing handy control in in vitro biological studies like anti-inflammatory and antidiabetic activity. Since the nanocomposite action and mechanism are totally different from others, like simple molecules of antibiotics. This work described a facile one-pot hydrothermal synthesis of the rGO-MnO2 nanocomposite. The prepared samples were characterized and confirmed by XRD, UV, SEM, EDX, and XPS studies. Furthermore, the catalytic performance evolved through in vitro biological applications such as anti-inflammatory and antidiabetic activities. rGO-MnO2 nanocomposite has more biological activity than pure MnO2 nanoparticles, as demonstrated by the results.