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Background: Perineal wounds are at risk for infection, without a good treatment, perineal wound can harbor sepsis and become a chronic, long-term problem. Binahong leaves is considered as an alternative to accelerate perineal wound healing process. Objective: To determine the effect of binahong leaf extract (anredera cordifolia (tenore) steen) on perineal wound healing process in postpartum mothers. Methods: This study employed a quasy experimental study with control group posttest-only design. The study was conducted in the working area of Telogosari Wetan Health Center and Health Center of Kulon Kota Semarang in January-February 2017. There were 36 respondents selected using purposive sampling assigned in the intervention group (Binahong leaves extract) and the control group (Povidon Iodine 10%). REEDA scale was used for assessing perineal healing. Data were analyzed using Mann Whitney, Chi Square and Kruskall Wallis test. Results: Findings showed that the average day of perineal wound healing process in the binahong leaves group was 6.11 days and in the povidon iodine group was 7.66 days. There was a statistically significant difference in the duration of wound healing between the experiment and control group with p-value 0,001 (p value <0.05). Conclusion: Binahong leaves extract is effective in healing perineal wound in postpartum mothers. Therefore, binahong leaves are recommended to use as an alternative treatment for the mothers.