Find in Library

Genetically modified (GM) animals have attracted considerable attention and faced challenges. Human serum albumin (<i>HSA</i>) GM goats have been used to produce goat milk with serum albumin from humans, which has shown great potential in the market. Establishing an accurate method to distinguish goats with a genome modified by <i>HSA</i> has become necessary. Here, we established a platform to detect <i>HSA</i> GM goats by combining the advantages of recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) strategies. The whole detection process could be completed within 1 h, obtaining a direct result that could be visualized by a characteristic red band after a quick amplification under a constant temperature of 42 °C in the RPA experiments. Moreover, the GM goats could be identified with a detection limit of 0.1 ng using our method. Therefore, this study provided a rapid and convenient RPA-LFD method for the immediate detection of <i>HSA</i> GM goats. This will be useful for the identification of <i>HSA</i> GM goats, which may be used to distinguish the mixture of GM mutton and normal mutton.