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We sequenced almost complete mitochondrial genome of Apis dorsata (Insecta: Hymenoptera: Apocrita: Apidae) with a length of 15,933 bp. The mitogenome sequenced harbours 13 protein-coding genes (PCGs), 21 transfer RNA genes (tRNA), two ribosomal RNA genes (rRNA), and one control region (D-loop) with very high AT-base content. The genome has similar codon usage and gene organization to those of mitogenome reported for other Hymenoptera. Phylogenetic analyses showed that Apis dorsata and (Apis cerana+Apis mellifera) are the sister taxa, and more original, which is widely accepted view.