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Wine has long been under the view of researchers to test it for prospective benefits to human health. Many recent studies have claimed how moderate consumption of wine, can be beneficial for us. The aim of this study was to assess antagonistic activity and probiotic potential of the LAB strains, Lactobacillus brevis, Lactobacillus hilgardii and Lactobacillus plantarum, isolated from wine against pathogenic bacteria. The antimicrobial activity was detected with disc diffusion method against five species of Gram-negative bacteria: Escherichia coli CCM 3988, Klebsiella pneumoniae CCM 2318, Salmonella enterica subsp. enterica CCM 3807, Shigella sonei CCM 1373, Yersinia enterocolitica CCM 5671 and five Gram-positive bacteria: Bacillus thuringiensis CCM 19, Enterococcus faecalis CCM 4224, Listeria monocytogenes CCM 4699, Stapylococcus aureus subsp. aureus CCM 2461, Streptococcus pneumonia CCM 4501. These results showed antagonistic activity and probiotic potential of pathogenic bacteria isolated from wine. The cell free supernatants (CFS) from Lactobacillus brevis, Lactobacillus hilgardii and Lactobacillus plantarum inhibited the growth of pathogenic bacteria.