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Objective: The present study has investigated the impact of Yoga Nidra in improving the wellbeing of an individual. Context: Thomas argued that there is no precise definition of well-being, and he argued that well-being is intangible, difficult to define, and even hard to measure. The impact of stress causes physical, cognitive, emotional, and behavioral disorders which result in deteriorating the well-being of an individual. Yoga Nidra relaxes the body at physical, mental, emotional, and spiritual levels. Methods: The comprehensive scale of well-being comprises these factors that are taken into consideration for assessing the well-being at these levels. The study investigated the well-being before and after the administration of Yoga Nidra intervention to the participants. This is a pre- and post test experimental study in which factors of well-being are being explored through extensive literature survey and instrument of well-being has been developed. The various factors were explored through exploratory factor analysis and validated it through confirmatory factor analysis. Results and Conclusion: The results showed that there is a significant influence of physical, mental, emotional, and spiritual components on well-being.