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Laron syndrome (LS) is a rare genetic disorder characterized by low levels of insulin-like growth factor 1 (IGF1) and high levels of growth hormone (GH) due to mutations in the growth hormone receptor gene (<i>GHR</i>). A <i>GHR</i>-knockout (<i>GHR</i>-KO) pig was developed as a model for LS, which displays many of the same features as humans with LS-like transient juvenile hypoglycemia. This study aimed to investigate the effects of impaired GHR signaling on immune functions and immunometabolism in <i>GHR</i>-KO pigs. GHR are located on various cell types of the immune system. Therefore, we investigated lymphocyte subsets, proliferative and respiratory capacity of peripheral blood mononuclear cells (PBMCs), proteome profiles of CD4⁻ and CD4⁺ lymphocytes and IFN-α serum levels between wild-type (WT) controls and <i>GHR</i>-KO pigs, which revealed significant differences in the relative proportion of the CD4⁺CD8α⁻ subpopulation and in IFN-α levels. We detected no significant difference in the respiratory capacity and the capacity for polyclonal stimulation in PBMCs between the two groups. But proteome analysis of CD4⁺ and CD4⁻ lymphocyte populations revealed multiple significant protein abundance differences between <i>GHR</i>-KO and WT pigs, involving pathways related to amino acid metabolism, beta-oxidation of fatty acids, insulin secretion signaling, and oxidative phosphorylation. This study highlights the potential use of <i>GHR</i>-KO pigs as a model for studying the effects of impaired GHR signaling on immune functions.