Generation of an iPS cell line from bone marrow derived mesenchymal stromal cells from an elderly patient
oleh: Matthias Megges, Sven Geissler, Georg N. Duda, James Adjaye
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2015-11-01 |
Deskripsi
An induced pluripotent stem cell line was generated from primary human bone marrow derived mesenchymal stromal cells of a 74 year old donor using retroviruses harboring OCT4, SOX2, KLF4 and c-MYC in combination with the following inhibitors TGFβ receptor-SB 431542, MEK-PD325901, and p53-Pifithrin α. Pluripotency was confirmed both in vitro and in vivo.