Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer
oleh: Marcus D. Wilson, Ludovic Renault, Daniel P. Maskell, Mohamed Ghoneim, Valerie E. Pye, Andrea Nans, David S. Rueda, Peter Cherepanov, Alessandro Costa
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-09-01 |
Deskripsi
Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into nucleosomal DNA, which together with single-molecule FRET measurements provides evidence for a DNA looping and sliding mechanism of integrases.