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<p>In Indonesia schistosomiasis is caused by Schistosoma japonicum with <em>O.h.lindoensis </em>snails as an intermediate host. For that we need to do research on the level of DNA polymorphism <em>O.h.lindoensis</em> snails. One method used is the technique of Random Amplified Polymerase ChainReaction (RAPD-PCR). Thereforewe need a proper method of DNA extraction aswell as in determining theoptimumreaction conditions forRAPD-PCR. This study aimed to be obtained of O.h.lindoensis DNA from in the highlands of Central Sulawesi in Bada, Napu and Lindu and to optimize condition of PCR-RAPD. To be obtained <em>O.h.lindoensis</em> DNA using with protocols or operating procedures PureLink Genomic DNA Mini Kits TM. The extraction of DNA yieled good quality of DNA. While amplification of DNA using PCR-RAPD will become efficient and consistent if the amplification reactions are in ideal condition. In <em>O.h.lindoensis</em>, clear PCR-RAPD patterns were obtained using 10ngDNAtemplate, 25 pmol primer and thenumberof thermal cycle was 40 x.</p>