Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results
oleh: W. Ray Waters, H. Martin Vordermeier, Shelley Rhodes, Bhagwati Khatri, Mitchell V. Palmer, Mayara F. Maggioli, Tyler C. Thacker, Jeffrey T. Nelson, Bruce V. Thomsen, Suelee Robbe-Austerman, Doris M. Bravo Garcia, Mark A. Schoenbaum, Mark S. Camacho, Jean S. Ray, Javan Esfandiari, Paul Lambotte, Rena Greenwald, Adrian Grandison, Alina Sikar-Gang, Konstantin P. Lyashchenko
| Format: | Article |
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| Diterbitkan: | BMC 2017-06-01 |
Deskripsi
Abstract Background Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.