Find in Library

<i>Perilla frutescens</i> var. <i>acuta</i> (Lamiaceae) is widely used not only as an oil or a spice, but also as a traditional medicine to treat colds, coughs, fever, and indigestion. As an ongoing effort, luteolin-7-<i>O</i>-diglucuronide (<b>1</b>), apigenin-7-<i>O</i>-diglucuronide (<b>2</b>), and rosmarinic acid (<b>3</b>) isolated from <i>P. frutescens</i> var. <i>acuta</i> were investigated for their anti-adipogenic and thermogenic activities in 3T3-L1 cells. Compound <b>1</b> exhibited a strong inhibition against adipocyte differentiation by suppressing the expression of <i>Pparg</i> and <i>Cebpa</i> over 52.0% and 45.0%, respectively. Moreover, <b>2</b> inhibited the expression of those genes in a dose-dependent manner [<i>Pparg</i>: 41.7% (5 µM), 62.0% (10 µM), and 81.6% (50 µM); <i>Cebpa</i>: 13.8% (5 µM), 18.4% (10 µM), and 37.2% (50 µM)]. On the other hand, the <i>P. frutescens</i> var. <i>acuta</i> water extract showed moderate thermogenic activities. Compounds <b>1</b> and <b>3</b> also induced thermogenesis in a dose-dependent manner by stimulating the mRNA expressions of <i>Ucp1</i>, <i>Pgc1a</i>, and <i>Prdm16</i>. Moreover, an LC-MS/MS chromatogram of the extract was acquired using UHPLC-MS² and it was analyzed by feature-based molecular networking (FBMN) and the Progenesis QI software (version 3.0). The chemical profiling of the extract demonstrated that flavonoids and their glycoside derivatives, including those isolated earlier as well as rosmarinic acid, are present in <i>P. frutescens</i> var. <i>acuta</i>.