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The paper presents a new method of producing the bio-oil from the algae Botryococcus branii type. There are several companies in the world that produce the micro-algae oil. These are methods of centrifuging the bio-oil produced from the algae, which destroys the algae. By using the appropriate of pulse power discharge, we can separate the bio-oil from the micro-algae without damaging their structure. After separation by pulse power discharges a algae remain in good condition and are capable of further the bio-oil production. This is very important as it is quite expensive to produce and maintain a algae in good condition, so there is no point in destroying them by centrifuging. Especially now it is a very important issue for bio-fuels in the current economic crisis.