Pairwise detection of site-specific receptor phosphorylations using single-molecule blotting
oleh: Kyung Lock Kim, Daehyung Kim, Seongsil Lee, Su-Jeong Kim, Jung Eun Noh, Joung-Hun Kim, Young Chan Chae, Jong-Bong Lee, Sung Ho Ryu
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-03-01 |
Deskripsi
Current methods to measure post-translational modifications of receptor tyrosine kinases provide ensemble results. Here the authors develop a single-molecule blotting (SiMBlot) assay that detects site-specific phosphorylation patterns at the single-molecule level.