Find in Library

Cytochrome P450 (CYP) is a group of important detoxification enzymes found in insects related to their resistance to insecticides. To elucidate the CYP6 family genes of P450, which are potentially related to imidacloprid resistance in <i>Aphis glycines</i>, the CYP6 cDNA sequences of <i>A. glycines</i> were studied. The transcriptome of <i>A. glycines</i> was constructed, and the CYP6 cDNA sequences of <i>A. glycines</i> were screened. Their relative expression levels in response to imidacloprid induction were examined through qRT-PCR, and the CYP6s with higher expression levels were used to study the detoxification of imidacloprid through RNA interference and a bioassay. Twelve CYP6s were obtained from the <i>A. glycines</i> transcriptome. These samples were named by the International P450 Nomenclature Committee and registered in GenBank. After 3, 6, 12, 24 and 48 h of induction with LC50 concentrations of imidacloprid, the relative expression levels of these CYP6s increased; the expression level of <i>CYP6CY7</i> experienced the highest increase, being more than 3-fold higher than that of those of the non-imidacloprid-induced CYP6s. After RNA interference for <i>CYP6CY7</i>, the relative expression level of <i>CYP6CY7</i> significantly decreased after 3, 6 and 12 h, while the corresponding P450 enzyme activity decreased after 12 and 24 h. The mortality of <i>A. glycines</i> due to imidacloprid treatment increased by 14.71% at 24 h. <i>CYP6CY7</i> might detoxify imidacloprid in <i>A. glycines</i>. This study provides a theoretical basis for the further study of the mechanism of action of CYP6s and potential new methods for improving insecticidal efficacy.