Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background

oleh: Karla F. Leavens, Catherine Osorio-Quintero, Elisabeth Ashlyne Yeuteuh, Francesca T. Perez-Profeta, Anna Ada Dattoli, Fabian L. Cardenas-Diaz, Deborah L. French, Paul Gadue

Format: Article
Diterbitkan: Elsevier 2024-12-01

Deskripsi

Over the past decade, the use of human stem cell-derived β cells (SC-β cells) to model pancreatic β cell development, function and disease has become increasingly common. Though protocols are rapidly improving, current directed differentiation strategies do not yield a pure population of insulin-positive SC-β cells in vitro. Therefore, it is experimentally advantageous to have reporter lines that allow for live sorting of insulin-positive populations. To aid in these studies, we have knocked mNeonGreen fluorescent protein into the endogenous insulin locus of the commonly used H1 (WA01) human embryonic stem cell line.