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Background: The purpose of the study was to evaluate the changes of light reflection% on two materials (monolithic zirconia and lithium disilicate) after using two external staining kits following by thermocycling. Methods: Specimens were sectioned from monolithic zirconia (n = 60) and lithium disilicate (<i>n</i> = 60) then divided into six groups (<i>n</i> = 20). Two different types of external staining kits were used and applied to the specimens. The light reflection% was measured before staining, after staining and after thermocycling using a spectrophotometer. Results: The light reflection% of zirconia was significantly higher compared to lithium disilicate at the beginning of the study (<i>p</i> = 0.005), after staining with kit 1 (<i>p</i> = 0.005) and kit 2 (<i>p</i> = 0.005) and after thermocycling (<i>p</i> = 0.005). For both materials, the light reflection% was lower after staining with Kit 1 compared to kit 2 (<i>p</i> < 0.043). After thermocycling, the light reflection% of lithium disilicate increased (<i>p</i> = 0.027) and was unchanged with Zirconia (<i>p</i> = 0.527). Conclusions: There is a difference between the materials regarding light reflection% as the monolithic zirconia showed higher light reflection% comparing lithium disilicate throughout the entire experiment. For lithium disilicate, we recommend using kit 1 as we found that, after thermocycling, the light reflection% of kit 2 was increased.