Bridge helix bending promotes RNA polymerase II backtracking through a critical and conserved threonine residue
oleh: Lin-Tai Da, Fátima Pardo-Avila, Liang Xu, Daniel-Adriano Silva, Lu Zhang, Xin Gao, Dong Wang, Xuhui Huang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-04-01 |
Deskripsi
RNA Polymerase II can detect and cleave mis-incorporated nucleotides by a proofreading mechanism that requires backtracking of the enzyme. Here the authors show that Pol II backtracking occurs in a stepwise mode that involves two intermediate states where the fraying of the terminal RNA nucleotide is a prerequisite.