Partial Purification and Characterization of Exo-Polygalacturonase Produced by <i>Penicillium oxalicum</i> AUMC 4153

oleh: Shamsan A. Almowallad, Ghedeir M. Alshammari, Muneer M. Alsayadi, Naofel Aljafer, Ekram A. Al-Sanea, Mohammed Abdo Yahya, Laila Naif Al-Harbi

Format: Article
Diterbitkan: MDPI AG 2022-02-01

Deskripsi

Pectinase enzymes are important industrial enzymes having considerable applications in several industries, especially in food processing. Pectinases contribute 25% of global food enzyme sales. Therefore, the demand for a commercial enzyme with desirable characteristics and low production costs has become one of the great targets. Hence, this study aims to produce exo-polygalacturonase (exo-PG) using local fungal isolate <i>Penicillium oxalicum</i> AUMC 4153 by utilizing sugar beet manufacturing waste (sugar beet pulp) as a sole raw carbon source under shaken submerged fermentation, which is purified and characterized to optimize enzyme biochemical properties for industrial application. The purity of the obtained exo-PG was increased by about 28-fold, and the final enzyme yield was 57%. The partially purified enzyme was active at a broad range of temperatures (30–60 °C). The optimum temperature and pH for the purified exo-PG activity were 50 °C and pH 5. The enzyme was stable at a range of pH 3 to 6 and temperature 30–50 °C for 210 min. The values for <i>K</i><sub><i>m</i></sub> and <i>V</i><sub><i>max</i></sub> were 0.67 mg/mL, with polygalacturonic acid as substrate and 6.13 µmole galacturonic acid/min/mg protein, respectively. It can be concluded that purified exo-PG production by <i>P. oxalicum</i> grown on sugar beet waste is a promising effective method for useful applications.