Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
oleh: Soo Y. Ro, Luis F. Schachner, Christopher W. Koo, Rahul Purohit, Jonathan P. Remis, Grace E. Kenney, Brandon W. Liauw, Paul M. Thomas, Steven M. Patrie, Neil L. Kelleher, Amy C. Rosenzweig
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-06-01 |
Deskripsi
The activity of the membrane-bound enzyme pMMO depends on copper but the location of the copper centers is still under debate. Here, the authors reconstitute pMMO in nanodiscs and use native top-down MS to localize its copper centers, providing insights into which sites are essential for activity.