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An experiment was conducted to identify the biochemical defense enzymes, viz. catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and total phenol responsible for resistance against A. solani in resistant (cv. H-88-78-1) and susceptible (Punjab Chhuhara) cultivars of tomato after the application of Amistar 25 SC (azoxystrobin 0.1%) during 2015 crop season. Azoxystrobin was applied for their efficacy in inducing defense enzymes in tomato against A. solani. Defense-related enzymes were increased in azoxystrobin pre-treated tomato plants. Early blight resistant tomato plants (cv. H-88-78-1) treated with novel QoI (Quinone oxidation inhibitor) fungicideazxoystrobin inoculated with A. solani under glasshouse conditions showed increased accumulation of defense enzymes, viz. CAT, POX, PAL and total phenol than control plants.