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Present investigation deals with the beneficial effect of Arbuscular Mycorrhiza (AM) fungal species Glomus fasciculatum on growth performance of Allium sativum under field condition. In AM inoculated garlic plant showed significant increase in plant growth parameters like plant height, total biomass and bulb diameters, bulb weight, and yield. There was 21.10 % increment in yield of Allium bulb as compared to non AM inoculated garlic plant. Percentage of AM colonization occurred at all sampling and the colonization was higher during bulb initiation. Mycorrhiza inoculated garlic plant showed more alliin and allinase enzyme activity as compared to non inoculated garlic plant. The results reveal that mycorrhiza contribute to growth and development of garlic plant under field condition.