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<i>Brassica napus</i> is the dominant oil crop cultivated in China for its high quality and high yield. The length of the main inflorescence and the number of siliques produced are important traits contributing to rapeseed yield. Therefore, studying genes related to main inflorescence and silique number is beneficial to increase rapeseed yield. Herein, we focused on the effects of <i>BnKAT2</i> on the main inflorescence length and silique number in <i>B. napus</i>. We explored the mechanism of <i>BnKAT2</i> increasing the effective length of main inflorescence and the number of siliques through bioinformatics analysis, transgenic technology, and transcriptome sequencing analysis. The full <i>BnKAT2</i>(<i>BnaA01g09060D</i>) sequence is 3674 bp, while its open reading frame is 2055 bp, and the encoded protein comprises 684 amino acids. <i>BnKAT2</i> is predicted to possess two structural domains, namely KHA and _CNMP-binding domains. The overexpression of <i>BnKAT2</i> effectively increased the length of the main inflorescence and the number of siliques in <i>B. napus</i>, as well as in transgenic <i>Arabidopsis thaliana</i>. The type-A <i>Arabidopsis response regulator</i> (<i>A-ARR</i>), negative regulators of the cytokinin, are downregulated in the <i>BnKAT2</i>-overexpressing lines. The <i>Aux/IAA</i>, key genes in auxin signaling pathways, are downregulated in the <i>BnKAT2</i>-overexpressing lines. These results indicate that <i>BnKAT2</i> might regulate the effective length of the main inflorescence and the number of siliques through the auxin and cytokinin signaling pathways. Our study provides a new potential function gene responsible for improvement of main inflorescence length and silique number, as well as a candidate gene for developing markers used in MAS (marker-assisted selection) breeding to improve rapeseed yield.