RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
oleh: Joseph Abatemarco, Maen F. Sarhan, James M. Wagner, Jyun-Liang Lin, Leqian Liu, Wafa Hassouneh, Shuo-Fu Yuan, Hal S. Alper, Adam R. Abate
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-08-01 |
Deskripsi
Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or streptavidin production.