A STRATEGY FOR THE RAPID IDENTIFICATION OF FUNGAL METABOLITES AND THE DISCOVERY OF THE ANTIVIRAL ACTIVITY OF PYRENOCINE A AND HARZIANOPYRIDONE
oleh: Laura P. Ióca, Stelamar Romminger, Mario F. C. Santos, Karin F. Bandeira, Fabiana T. Rodrigues, Miriam H. Kossuga, Karen J. Nicacio, Everton L. F. Ferreira, Raquel P. Morais-Urano, Messias S. Passos, Luciana K. Kohn, Clarice W. Arns, Lara D. Sette, Roberto G. S. Berlinck
| Format: | Article |
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| Diterbitkan: | Sociedade Brasileira de Química 2016-07-01 |
Deskripsi
The isolation and identification of bioactive metabolites from complex extracts obtained from microbial growth media is a time consuming, costly, and labor-intensive task. A strategy to rapidly identify secondary metabolites isolated from extracts obtained from the culture media of marine-derived and endophytic fungal strains is described. Identification was achieved by HPLC-UV-MS and 1H NMR analyses in combination with data obtained from the Dictionary of Natural Products. Among the compounds identified, (-)-naphthoquinoneimine, citreorosein, emodin, pyrenocine A and harzianopyridone displayed moderate to potent antiviral activity. (-)-Naphthoquinoneimine was isolated as the enantiomer of its previously reported dextrorotatory congener, while 6,7-dihydroxy-2,2-dimethyl-4-chromanone is herein reported for the first time as a natural product.