Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification
oleh: Fan Liu, Philip Lössl, Richard Scheltema, Rosa Viner, Albert J. R. Heck
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-05-01 |
Deskripsi
Chemical cross-linking combined with mass spectrometry (XL-MS) can provide information on protein conformations and interactions in highly complex samples. Here the authors describe an improved XL-MS workflow to increase the depth and fidelity of cross-link identification using whole proteome databases.