A vaccinology Approach to the Identification and Characterization of <i>Dermanyssus Gallinae</i> Candidate Protective Antigens for the Control of Poultry Red Mite Infestations
oleh: José Francisco Lima-Barbero, Marinela Contreras, Lourdes Mateos-Hernández, Francisco Manuel Mata-Lorenzo, Roxana Triguero-Ocaña, Olivier Sparagano, Robert D. Finn, Christina Strube, Daniel R.G. Price, Francesca Nunn, Kathryn Bartley, Ursula Höfle, Mariana Boadella, Alasdair J. Nisbet, José de la Fuente, Margarita Villar
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2019-11-01 |
Deskripsi
The poultry red mite (PRM), <i>Dermanyssus gallinae</i>, is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate protective antigens. Based on proteomic data from fed and unfed nymph and adult mites, we selected a novel PRM protein, calumenin (Deg-CALU), which is tested as a vaccine candidate on an on-hen trial. <i>Rhipicephalus</i> <i>microplus</i> Subolesin (Rhm-SUB) was chosen as a positive control. Deg-CALU and Rhm-SUB reduced the mite oviposition by 35 and 44%, respectively. These results support Deg-CALU and Rhm-SUB as candidate protective antigens for the PRM control.