Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
oleh: Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov, Mark Howarth
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-04-01 |
Deskripsi
Peptide tags are important for protein purification but have minimal benefits afterwards. Here the authors present Spy&Go, which uses rational engineering of the SpyDock protein to capture SpyTag peptides without requiring an irreverisble isopeptide bond.