Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
oleh: Sean P. Carney, Wen Ma, Kevin D. Whitley, Haifeng Jia, Timothy M. Lohman, Zaida Luthey-Schulten, Yann R. Chemla
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-12-01 |
Deskripsi
UvrD is a model helicase from the non-hexameric Superfamily 1. Here, the authors use optical tweezers to measure directly the stepwise translocation of UvrD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but sequesters the nascent single strands, releasing them after a variable number of ATP hydrolysis cycles.