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In this paper, based on field, herbarium and bibliographic research, new distributional data for 16 species and subspecies (13 natives and 3 aliens) are reported for Ventotene and Santo Stefano islands. Particularly, 6 are new or confirmed to Ventotene and 8 to Santo Stefano. One species is excluded from the flora of Ventotene and one is to be considered locally extinct. One native and one alien are new to Lazio administrative region.