PhotoGate microscopy to track single molecules in crowded environments
oleh: Vladislav Belyy, Sheng-Min Shih, Jigar Bandaria, Yongjian Huang, Rosalie E. Lawrence, Roberto Zoncu, Ahmet Yildiz
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-01-01 |
Deskripsi
Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.