Flow cytometric discrimination of seven lineage markers by using two fluorochromes.
oleh: Francesco Boin, Maria Letizia Giardino Torchia, Ivan Borrello, Kimberly A Noonan, Matthew Neil, Mark J Soloski, Raffaello Cimbro
| Format: | Article |
|---|---|
| Diterbitkan: | Public Library of Science (PLoS) 2017-01-01 |
Deskripsi
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.